Samples of frozen tissues were thawed, weighed and homogenized in phosphate buffered saline, pH 7.4 (PBS) to give 10% (brain) or 20% (lymphoid tissues) w/v homogenates. Western blotting for PrPSc detection was performed following proteinase K digestion and sodium phosphotungstic acid (NaPTA) precipitation of tissue homogenates, as previously described [27], using the primary anti-PrP monoclonal antibodies ROS-BC6 (raised against recombinant sheep PrP residues 94–233; epitope amino acids 144FGNDYEDRYYR154) and ROS-IH9, both at 0.5 μg/ml [25].

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.