Signal peptide and transmembrane domain were predicted using SignalP4.1 [94] and SMART [95], respectively. Deduced amino-acid sequences of Phh-GluCl and Phh-RDL were aligned using the MUSCLE algorithm [96] and further processed with GeneDoc (IUBio). The phylogenetic distance trees were generated on amino-acid sequences by the SeaView software [97] using BioNJ Poisson parameters and bootstrap values were calculated with 1000 replicates as described previously [98]. The resulting trees were modified by FigTree (http://tree.bio.ed.ac.uk/software/figtree/). The accession numbers for the protein sequences mentioned in this article are: Apis mellifera (Ame): GluCl ABG75737, GRD AJE68942, LCCH3 AJE68943, RDL AJE68941, CG8916 NP_001071290; nAChR7 AJE70265; Ctenocephalides felis (Cfe): RDL AHE41088; Drosophila melanogaster (Dme): GluCl AAC47266, GRD NP_524131, LCCH3 NP_996469, RDL NP_523991, CG8916 NP_001162770; Ixodes scapularis (Isc): GluCl ALF36853; Musca domestica (Mdo): GluCl BAD16657, RDL NP_001292048; Pediculus humanus humanus (Phh): GluCl1 MT321070, GluCl2 MT321071, RDL1 MT321072, RDL2 MT321073; Rhipicephalus microplus (Rmi): GluCl AHE41097, RDL AHE41094; Tetranychus urticae (Tur): GluCl BAJ41378 and Tribolium castaneum (Tca): GluCl NP_001107775, GRD NP_001107772, LCCH3 NP_001103251, RDL NP_001107809, 8916 NP_001103425.

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