The colony of human body lice (Pediculus humanus humanus) were provided by Kosta Y. Mumcuoglu from the Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel. This laboratory-reared colony adapted to rabbit blood were fed four times per week for 20–30 min on rabbits and maintained at 30 ± 1°C and 60–70% relative humidity without exposure to any drugs [90]. Survival and immobilization were monitored by microscopical observations at 1, 2, 3 and 24 h after contact of 44 lice at different stages of the life cycle with insecticides at concentrations ranging from 1 to 100 μM or 1% DMSO on a Whatman grade 1 filter paper in a 55 mm Petri dish following the knockdown classification from KD+ to KD++++ (S1 Table), as previously described [12]. Nits (50 to 190 per condition) were incubated with lotilaner or ivermectin and hatching was monitored between six and nine days. Water and solution of 1% DMSO were used as negative controls. Pictures were recorded with a Motic Digital Microscope 143 Series and the Motic Image Plus 3.0 software.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.