Circular dichroism (CD) and transmission electron microscopy (TEM)

These assays were performed according to a previously reported protocol [34]. Samples consisted of 5 μM PrP in solution or with 10 μg/mL or 30 μg/mL heparin were incubated at 25°C for 5 h. The pellet was separated by centrifugation (14,000 g, 30 min) and the supernatant was used for CD analysis. The ellipticity values (MilliQ water or heparin solution) were used as controls. For TEM, 4 μL incubating solution not centrifuged was fixed on 300 mesh copper grids (BZ10023b, Zhongjingkeyi), washed with 4 μL water, negatively stained using 2% uranyl acetate and examined on a Tecnai G2 Spirit TEM at voltage of 120 kV.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.