Circular dichroism (CD) and transmission electron microscopy (TEM)

These assays were performed according to a previously reported protocol [34]. Samples consisted of 5 μM PrP in solution or with 10 μg/mL or 30 μg/mL heparin were incubated at 25°C for 5 h. The pellet was separated by centrifugation (14,000 g, 30 min) and the supernatant was used for CD analysis. The ellipticity values (MilliQ water or heparin solution) were used as controls. For TEM, 4 μL incubating solution not centrifuged was fixed on 300 mesh copper grids (BZ10023b, Zhongjingkeyi), washed with 4 μL water, negatively stained using 2% uranyl acetate and examined on a Tecnai G2 Spirit TEM at voltage of 120 kV.

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