Data are expressed as mean ± SEM. The normal distribution was verified by the Shapiro-Wilk test. Comparisons among the groups were performed by unpaired Student’s t-test, one-way ANOVA or two-way ANOVA followed by the Tukey or Sidak post hoc analysis, whenever applicable. Statistical significance was set as p < 0.05. The statistical package used was GraphPad Software’s Prism 6®.

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