In summary, bacterial suspensions containing the microorganism Lactobacillus casei Shirota (Yakult Brasil Ltda, Lot # 0758) were used in the experiments. This strain was chosen because it is a bacterial species that poses no risk of environmental contamination and measures 0.5 μm (the SARS-CoV-2 virus measures 0.1 μm). Additionally, this microorganism has already been tested and validated for the dispersion model in a dental clinic environment in a previous study [8]. Thus, a viability test was performed to determine the initial concentration of 1.5x108 CFU / mL of L. casei Shirota, based on the study by Marthi et al. [17].

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