VO2 was measured via open-flow indirect calorimeter (LE400, Panlab, Harvard Apparatus, Cornella, Spain) calibrated with a certified gas mixture (high O2 = 50.05%, high CO2 = 1.51%, low O2 = 20.02% and low CO2 = 0.00%). The air flow rate established was equivalent to 0.6 L min–1 throughout the procedures. VO2 data were analyzed using a computerized system (Metabolism software version 2.2.01 Panlab, Harvard Apparatus), transformed to milliliters per minute and relativized by the mice body mass (mLO2 kg–0.75 min–1).

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