VO2 was measured via open-flow indirect calorimeter (LE400, Panlab, Harvard Apparatus, Cornella, Spain) calibrated with a certified gas mixture (high O2 = 50.05%, high CO2 = 1.51%, low O2 = 20.02% and low CO2 = 0.00%). The air flow rate established was equivalent to 0.6 L min–1 throughout the procedures. VO2 data were analyzed using a computerized system (Metabolism software version 2.2.01 Panlab, Harvard Apparatus), transformed to milliliters per minute and relativized by the mice body mass (mLO2 kg–0.75 min–1).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.