Wild-type C57BL/6 male mice were allocated into three groups, rest, low intensity (40% of Speak), or high intensity (80% of Speak) of exercise on the treadmill to analyze oxygen consumption (VO2) (LE400, Panlab, Harvard Apparatus, Cornella, Spain). The exercise intensity was calculated from Speak achieved in the incremental speed-running test until fatigue. Each exercise or rest lasted 30 min. The slope of the treadmill was maintained at 5°. All these procedures were performed at least 48 h after the incremental-speed running test until fatigue.

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