Live imaging was performed using Zeiss Observer D1 with Axio-Cam. Fixed cells were analyzed by Olympus FV1000 or Zeiss LSM 880 confocal microscope as well as Keyence BZX 800 for overview images. For retinal cross sections, two areas/section with 625 μm × 625 μm dimension at 200 × magnification, four optical sections of 2 μm thickness, for two sections per mouse, of at least six light damaged and undamaged controls were counted. For Otx2 cell assessment in the ONL, vertical rows of cells were counted for six different areas per image. Values are expressed as mean ± standard deviation (S.D.). Statistical analyses were performed by Mann-Whitney (U) test for independent samples and the Wilcoxon test for dependent samples. Holm-Bonferroni method was used to correct for multiple comparisons.

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