All retinas were checked for successful recombination under the fluorescence microscope before every sort. For each sort, about 6–10 retinas were pooled and dissociated in DNase/Papain (75 μl/ 750 μl, respectively, Worthington) for 20 min at 37°C on the shaker, triturated, mixed with Ovomucoid (750 μl), centrifuged for 10 min at 300 × g and resuspended in 800 μl DNase/ Ovomucoid/ Neurobasal solution (1: 1: 10, respectively, Gibco) per retina. Cells were filtered through a 35 μm filter, sorted using an 85 micron nozzle, and collected into two chilled tubes. Cell sorts were performed using BD Aria III cell sorter (BD Bioscience). Debris was excluded from the sort and only all events in gate P1 were sorted (Supplementary Figure 1A). Cells with the brightest fluorescence were found in gate P3 (“positives,” MG fraction), cells with no fluorescence in gate P2 (“negatives,” neuronal fraction, Supplementary Figure 1A′), everything in between was excluded. Gating settings were kept throughout all sorts for undamaged and damaged retinas. The fraction of MG comprised about 1.7% Supplementary Figures 1A″, A). Samples were collected in FBS-coated tubes containing Neurobasal medium. After collection, the tdTomato+ MG fraction (P3) and the tdTomato fraction (P2) was post-sorted to validate purity (Supplementary Figures 1B–B″,C–C″). Cells were spun for 10 min at 300 × g at 4°C, the pellet was homogenized in Qiazol (Qiagen) and stored at −80°C.

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