All mice were housed at the State University of New York, College of Optometry and used in accordance with the Institutional Animal Care and Use Committee approved protocols (IACUC). Rlbp1-creERT2 mice (obtained from Dr. Edward Levine, S129 background) were crossed to R26-stop-flox-CAG-tdTomato mice (Jackson Labs, also known as Ai14, #007908) and will be henceforth referred to as RlbpCreER: stopf/f-tdTomato or wild type (wt). For light damage mice, Rlbp1-creERT:tdTomato mice were crossed to the albino Swiss Webster mouse (CSW 024, Charles River Laboratories) that carries the RPE65450Leu gene (confirmed by genotyping). In addition, the Hes5-GFP mouse (Basak and Taylor, 2007) was used as another MG-specific reporter mouse established in the lab (Nelson et al., 2011). The Hes5 mouse (S129 background) was also crossed to the Swiss Webser mouse. Genotyping was done using the primers listed in Supplementary Table 1. For the detection of RPE65 variants, a subsequent digest of the PCR product with the restriction enzyme MwoI was performed for 2 h at 37°C. Tamoxifen (Sigma, St. Louis, MO) was administered intraperitoneally at 75 mg/kg in corn oil for four consecutive days in adult mice (2–3 months of age) to initiate the recombination of the floxed alleles.

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