Eye movement recordings were carried out with OSCANN, an eye-tracking device based on video-oculography technology (Hernández et al., 2018). Stimuli were bright green dots with a diameter of two centimeters presented on a display at a viewing distance of 60 centimeters. An examination protocol establishing the sequence of tests and standardized instructions for participants was followed to minimize variability as much as possible. Definite trials were preceded by practice trials that allowed confirming the understanding of the tests. Each definite test was preceded by a nine point-calibration and began with a central fixation target. The evaluation comprised a prosaccade test, an antisaccade test, a memory saccade test and a sinusoidal smooth pursuit test. The prosaccade, antisaccade and memory saccade tests included 12 trials in the horizontal plane followed by eight trials in the vertical plane each. Horizontal trials consisted of the random appearance of targets at 5, 10, or 20 degrees to right or left; and, in vertical trials, at 5 or 12 degrees up or down. The sinusoidal smooth pursuit test included six horizontal trials and six vertical trials.

Prosaccades were evaluated by the random appearance of an eccentric target, subsequently replaced by the reappearance of the central target. Subjects were instructed to keep their gaze fixed on the target.

Here, the target moved from one end of the screen to the other and subjects were asked to follow it as accurately as possible.

In a similar fashion to that in the prosaccade test, the central target was replaced by the appearance of an eccentric target, but the command, in this case, was: “When the target appears at one side, look at the opposite location, in a mirrored way. If you realize that you have looked at the target, try to correct yourself looking at the opposite location.”

As in the prosaccade test, the target appeared eccentrically and then at the central position. After that, the target disappeared, leaving the screen blank. The instructions were: “Keep your gaze fixed on the target when it appears at one side and when it comes back to the center. When the central target disappears, look at the location where it had previously appeared.”

In order to use the subtle alterations of eye movements for diagnostic aims, it is crucial to guarantee the reproducibility of the measuring, which is described in the OSCANN medical device user manual and summarized in a related publication (Hernández et al., 2018). After the automatic analysis of the images captured by the eye-tracker camera, we extracted features from each oculomotor test following the published methodology (García Cena et al., 2020).

Oculomotor responses can be characterized by diverse parameters. For descriptive purposes, such parameters have been grouped into three domains: (a) parameters related to spatial accuracy, as saccade error (the deviation of the final position of the gaze from the target, measured as positive or negative error) and pursuit error (the difference between the target position and the gaze position during a pursuit test); (b) parameters related to time, as latency (defined by the time delay between the appearance of a peripheral target and the onset of the ocular movement) and pursuit gain (the rate between ocular velocity and target velocity during a pursuit test); and (c) parameters related to success, as the percentage of correct memory saccades in the memory saccade test, and, in the antisaccade test, the percentage of correct antisaccades, corrected erroneous antisaccades (henceforth, corrected antisaccades) and successful antisaccades, which represent the sum of correct and corrected antisaccades. Precise definitions can be found in the Supplementary Material.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.