The AFS device consists of two glass layers hosting a microfluidic channel whose thickness and width are 100 μm and 2 mm, respectively. A transparent piezoelectric element glued on top allows the imaging of the sample in transillumination mode. The piezoelectric element was driven with a function generator in combination with an RF-amplifier to resonantly excite a planar acoustic standing wave over the chamber. A more detailed description of the AFS module developed by LUMICKS can be found in Kamsma et al. (2016). The resonant acoustic wave was exploited to exert forces over a range of pNs-nNs on hundreds of silica microspheres (diameter, 9.2 μm, Cospheric, Cat. No. SS05003) in parallel and with sub-millisecond response time. Data were acquired using the LUMICKS AFS technology including a LabVIEW interface dedicated for microsphere tracking with an integrated temperature controller (AFS-TC, LUMICKS).

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