Cell pellets were obtained for RNA from feeder-dependent conditions using dispase (0.2 μg/mL; Gibco), allowing the colonies to settle, and rinsing twice in DPBS to remove as many MEFs as possible or from feeder-free conditions using Accutase. RNA was then extracted from these pellets using the mirVana™ miRNA Kit (Ambion; Invitrogen) according to the supplied protocol.

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