When the cell confluence reached about 80%, the pcs2+-miR-24b-3p, pcs2+-lnc017705, and pmir-GLO-ZIP1-3′ UTR were co-transfected into HEK293T cells. Similarly, the pcs2+-miR-24b-3p and pmir-GLO-lnc017705 were co-transfected into cells. After incubation for 24 h, the cells were collected and lysed, and dual luciferase activity was measured using a dual luciferase assay kit (Vazyme, Nanjing, China) and an automatic microplate reader (Molecular Devices, Sunnyvale, USA). The firefly luciferase activity was normalized against Renilla luciferase activity.

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