NWR iPSCs were differentiated into cardiomyocytes using a human kit (PSC Cardiomyocyte Differentiation Kit:Gibco), using the supplied protocol. In brief, feeder-free iPSCs were plated at a density of 6,400–7,900 cells/cm2 on Geltrex in mTeSR1. The use of Rho kinase (ROCK) inhibitors was not necessary for cell survival during plating. Successful wells achieved ∼20% confluency on the first day of differentiation, slightly less than the minimum of the range of 30%–90%. Medium was changed every other day, and cells began to contract ∼9 days after the start of differentiation.

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