The whole lengths of lnc017705 and the dre-miR-24b-3p gene were cloned into the overexpression vector pcs2(+). The ZIP1 3′ UTR including the binding site of miR-24b-3p was amplified and inserted into the pmir-GLO vector (Promega, Madison, WI, USA) at the 3′ end of the luc2 (firefly luciferase) gene (pmir-GLO-ZIP1-3′ UTR). Similarly, the vectors of pmir-GLO-lnc017705 were obtained using the same method. Primer sequences were shown in Supplementary Table 1. All constructs were verified by sequencing.

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