For validation of AS isoforms, five isoform genes were randomly selected, and gene-specific primers were designed using NCBI Primer-BLAST, and the expression profiles of nine lncRNAs and nine miRNAs were verified using qPCR technology. The PCR products of AS isoforms mixed with 10 × loading buffer and GelRed fluorescent nucleic acid dyes were electrophoresed in 1.4% agarose gel for 1 h. Real-time qPCR was performed to validate gene expression using TB Green® Fast qPCR Mix on the QuantStudio™ 6 Flex qRT-PCR system (ABI, Germany). The specific primer pairs of the validated genes were shown in Supplementary Table 1. The relative expression of the genes was calculated based on the comparative CT (2−ΔΔCT) method. Statistical analysis was performed using SPSS software. Data were presented as mean ± standard deviation (SD), with a statistical significance of P < 0.05.

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