The Sendai virus appeared to have little to no cytotoxicity for NWR fibroblast cells, which we needed to account for during the initial seeding, as severe overgrowth of the cells frequently occurred. Clearance of the Sendai virus from the cells was tested using the published primer sequences provided in the kit instructions. Positive controls were collected during the replating on day 5 and compared to the iPSCs collected at passage 6, 11, 16, and 21.

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