This rhinoceros iPSC clone was also successfully adapted to feeder free conditions using mTeSR™1 (Stem Cell Technologies) medium and the Geltrex substrate. Previous attempts with less nutrient rich and more defined media such as mTeSR-E8™ (Stem Cell Technologies) failed to support growth of the rhinoceros cells. Cells grown in feeder-free conditions were passaged using StemPro® Accutase® (Gibco).

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