Physical properties and size distribution of hydrogel microspheres
This protocol is extracted from research article:
A Versatile Microencapsulation Platform for Hyaluronic Acid and Polyethylene Glycol
Tissue Eng Part A, Feb 15, 2021; DOI: 10.1089/ten.tea.2019.0286

Images of individual microspheres were captured using a Biotek Cytation 5 Multi-mode imaging reader. The microspheres were imaged in multiwell plates in phosphate-buffered saline (PBS), and the images were analyzed in Adobe Photoshop to determine the average microsphere diameter and size distribution for each microsphere type (N = 100 microspheres/group). Hydrogel microspheres were further characterized by determination of the swelling ratio, “Q,” for each microsphere type, which is the ratio of the swollen hydrated mass to the dry mass.28 Microspheres were immersed in excess DI water for 24 h before measurement to remove dissolved salts and to ensure equilibrium swelling was reached. Microspheres were removed of excess surface moisture and then weighed on a preweighed watch glass. Subsequently, the spheres were dried overnight at 60°C and reweighed to obtain the dry mass (N = 3/group).

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