Bacterial DNA was extracted from the film dressings as described previously [12, 34]. Briefly, using a safety cabinet, each film dressing was minced by sterile scissors, followed by chemical (1.2% Triton-X 100) and enzymatical (lysozyme and lysostaphin) disruption of the bacterial cell walls. The resultant solution was then cleaned-up using a QIAamp DNA Mini Kit (QIAGEN, Venlo, The Netherlands) according to the manufacturer’s instructions. Each extracted bacterial DNA sample was recovered in 100 μL of AE buffer (10 mM Tris-HCl, 0.5 mM ethylenediaminetetraacetic acid, pH 9.0). The DNA solution was stored at − 20 °C until analysis.

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