Biotin-coupled miRNA mimics (GenePharma) for miR-411 (Bio-miR-411 and Bio-miR-411-MUT) and miR-1205 (Bio-miR-1205 and Bio-miR-1205-MUT) were respectively transfected into HCT116 and CaCo-2 cells, with Bio-miR-NC as the negative control. Whereafter, cells were lysed and incubated with Pierce™ Streptavidin Magnetic Beads (Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C overnight. Total RNA was purified from the magnetic beads and circ_0082182 enrichment was assayed by quantitative real-time polymerase chain reaction (qRT-PCR).

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