Hematoxylin and eosin staining
This protocol is extracted from research article:
Lotus leaf extract inhibits ER− breast cancer cell migration and metastasis
Nutr Metab (Lond), Feb 18, 2021; DOI: 10.1186/s12986-021-00549-0

For histopathology analysis, lung and liver tissues were fixed by paraformaldehyde and then were dehydrated, embedded in paraffin and cut into serial sections at 5 μm. Sections were stained with hematoxylin and eosin (H&E) solution and observed them under an optical microscope (DP73, OLUMPUS, Japan).

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