HCT116 and CaCo-2 cells were seeded into the 6-well plates. Two vertical scratches were scraped by a sterile pipette tip in single-layer cells. The waste cells were removed by PBS (Gibco), and cell culture was performed in DMEM + 10% FBS for 24 h. Cells were photographed at 0 h and 24 h under a microscope (× 40 magnification). The scratch width was measured and the migration rate (%) was calculated using the following formula: (scratch width at 0 h − scratch width at 24 h)/scratch width at 0 h.

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