Cells were seeded in dish to form a confluent monolayer. Then 200 μl pipette tip was used to make a scratch in the middle of plate, and wash twice with PBS. The monolayer was maintained with the FBS-free media with LAE supplement. Wound closure was monitored after 0 and 36 h. The percent of wound closed are calculated by image J. TGF-β1 was purchased from Peprotech (USA, # 100-21-10) and dissolved in 10 mM citric acid, pH3.0 at 0.1–1.0 mg/ml. Chloroquine diphosphate (CQ) was purchased from Medchem Express (Shanghai, China, cat# HY-17589) and dissolved in H2O to 10 mg/ml. LiCl was purchased from Sigma-Aldrich (Sigma, St. Louis, MO, USA, # 213233) and dissolved in cell culture medium to 20 mM. Nuciferine was purchased from Medchem Express (Shanghai, China, cat# HY-N0049) and dissolved in DMSO to 1.6925 mM.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.