Two hundred cells in the 6-well plates were cultured in the 37 °C incubator for 2 weeks until the colonies were observed. These colonies were fixed and stained using 4% Paraformaldehyde Fix Solution and Crystal Violet Staining Solution (Beyotime), followed by the manual counting of colonies.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.