In a previous research [22], the mRNA profiles in the liver (n = 5) and adrenal glands (n = 5), including the same samples of liver and adrenal glands sequenced by strand-specific transcriptome sequencing in the current study, were analyzed using the RNA-seq technique. Briefly, the library was sequenced in a paired-end reads modus of 150 bp per read using Illumina® HiSeq 2000 platform. Furthermore, in this previous study, genome-wise false discovery rate (FDR), termed as q-value was calculated for differential expression analysis, and genes with q < 0.05 were selected as DEGs [22].

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.