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The ZDHS study area was Zimbabwe which has one of the highest burden of HIV in the world, with approximately 1.2 million persons aged 15–64 years old living with HIV in 2016 [24]. The main source of secondary data for this study was the most recent ZDHS [25]. Figure 2 shows the geographic locations utilized for sample collection.

Zimbabwe Demographic and Health Survey (ZDHS) sample locations (blue dots). Maps were created using ArcGIS® software by Esri version 10.3 (

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