The genetic distance (GD) of SSR markers between parents was determined according to Nei’s et al. by Powermarker 3.25 [39, 40]. The formula is GDSSR = 1-2Nab/(Na + Nb), where Nab represent the SSR marker numbers amplified in both sample a and b, and Na and Nb represent amplified SSR marker numbers in sample a and b, respectively. The GD of SNP markers between parents were calculated by TASSEL 5.0 based on the identity-by-state (IBS) genetic distance as GDSNP = 1-IBS [41]. The average performance of MPH and BPH of ten yield and fiber quality related traits of 1128 F1s were analyzed by Graphpad prism 7.0. The packages ggplot2 and GGally in R software were used to analyze the correlation between GD of SSR and SNP marker and F1s performance, MPH, and BPH. Pearson’s correlation coefficients (r) were used to analyze the correlation between parent traits and F1s performance, MPH, and BPH and tested at P = 0.05 and 0.01.

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