In a previous research [22], a total of 99 eight-week-old pathogen-free female Sprague-Dawley rats (Beijing Vital River Laboratory Animal Technology Co., Ltd., Beijing, China), weighing 205 ± 7.16 g, were used to establish HS model under various HS conditions. Eighteen of them were assigned to the HS treatment (H120; 42 °C for 120 min). Ten rats, including 5 rats from Control group and 5 from the H120 group were used to perform the RNA-seq. Based on the PCC of samples, 3 rats in each group were selected for lncRNA-seq. Prior to the beginning of the HS experiment, all experimental rats were housed (3 rats per cage) at 22 ± 1 °C and 50% room humidity with the light/dark cycle (on 06:00 AM, off 18:00 PM) for 1 week. During the whole experiment, rats were given access to feed and water ad libitum. Moreover, the experiment was performed in the floor-standing artificial climate incubator (BIO250, BOXUN Medicine Instrument Co, Shanghai, China). Rats in the Control group were never introduced to the incubator and were placed at room temperature. The experiment was conducted with healthy and conscious rats.

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