For activity of the brush border enzymes, lactase, maltase, sucrase, aminopeptidase N (ApN), aminopeptidase A (ApA), dipeptidylpeptidase IV (DPPIV) tissue samples from the mid small intestine were homogenized in 1% Triton X-100 buffer using a gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Assays were performed using specific substrates, as described previously (33). Interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α levels in mid small intestine and colon homogenates were analyzed using DuoSet ELISA kits (R&D systems, Minneapolis, MN) following the manufacturer's instruction. Each sample was tested in duplicate and measurements below detection limits were assigned a value of zero for the given analyte.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.