Cells were fixed with 4% paraformaldehyde for 30 min, washed three times with PBS, and then incubated with 0.1% Triton X-100 for permeabilization. Following blocking with 5% BSA, cells were stained with rabbit anti-E-cadherin (1:200) and N-cadherin (1:200) polyclonal antibodies overnight at 4°C and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Abcam, ab150080) for 1 h. Images were captured using a fluorescent microscope (OLYMPUS, BX53).

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