The indicated cells treated with the tested compounds (0, 1, and 2 μM) for 24 h were harvested and washed with PBS, then resuspended and incubated with PI/Annexin-V solution (KeyGEN Biotech). Apoptosis was analyzed with FACSCalibur flow cytometer (sysmex, CyFlow@ Cube 6, Germany), and data were analyzed by FlowJo7.6.1 software.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.