The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT; Sigma, M2128) assay. Briefly, cells (3 × 103 cells/well) were seeded in 96-well plates for 24 h and after treatment, 10 μl of MTT was added to each well and incubated for 4 h. Subsequently, medium was removed and 200 μl of 100% DMSO was added to dissolve the crystal formazan dye. The absorbance value was then determined at 570 nm.

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