Leaves with signs of disease were surface sterilized by dipping in 0.5% sodium hypochlorite for 2 min, followed by three rinses in sterile distilled water. Small leaf sections were cut from the spot margin and individually macerated in 500 µL of sterile distilled water, using a sterile mortar and pestle. Resulting suspensions were streaked onto nutrient agar (NA; BD Difco Laboratories, Sparks Maryland, MD, USA) (Shaad, Jones & Chun, 2001) and plates incubated at 27 °C for 48 h, after which they were examined for bacterial colonies development. Pure cultures of bacterial strains were obtained by colony sub-culturing. Collected bacteria cultures were suspended in sterile 0.85% saline solution and stored at 4 °C, until use.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.