Sections of right kidney were subjected to routine tissue processing, then embedded in paraffin and sectioned (5 μm) for hematoxylin and eosin (H&E) staining. The slides prepared were examined in blinded manner, selecting five fields of renal cortex and medulla (200× magnification) at random to gauge the extent of morphologic changes. Renal injury (i.e., tubular vacuolar change, tubular necrosis, tubular casts, inflammatory cells, renal interstitial fibrosis, and tubular dilatation) was scored on a scale of 0–4 as follows: 0 (normal kidney); 1 (<5% of renal injury); 2 (5–25% of renal injury); 3 (25–75% of renal injury); and 4 (>75% of renal injury) (Ulusoy et al., 2014).

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