The threshold for SARS-CoV-2 positivity was set on the presence of more than 25 viral template copies per milliliter, which was calculated based on a SARS-CoV-2 dilution series of SCV2QC (Qnostics). The cut-off CT-values were determined for each platform separately. Results of samples with CT-values representing ≤25 viral copies per milliliter, but with a visible PCR signal, crossing the threshold, were assigned as ‘borderline’ test results. Samples were considered as being negative if no amplification occurred.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.