LAD tissue sections were dewaxed with xylene and hydrated with alcohol at descending concentrations. Following antigen retrieval, the tissues were incubated with goat polyclonal antibody to HOXA10 (1: 200; ab191470; Abcam) at 4 °C overnight, as well as with biotinylated rabbit anti-goat secondary antibody (ab97100; Abcam) at 37 °C for 30 min. Diaminobenzidine (Bost Biotechnology Co., Ltd., Wuhan, China) was added stained for 1–2 min, while hematoxylin (KeyGEN Biotech Co., Ltd., Nanjing, Jiangsu, China) was counterstained for 1 min. The tissue sections were subsequently dehydrated and fixed using neutral balm. Five fields of view were selected and observed under an optical microscope (200 ×, Nikon, Tokyo, Japan) with 100 cells from each field analyzed. Brown-yellow cells were considered to be positive. Cells with a staining degree greater than 25% were considered to be positive cells with the positive rate calculated using the following formula: positive rate = (the number of positive cells/the number of total cells) × 100%.

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