Eighteen six-week-old female athymic BALB/c nude mice (weight: 18–20 g) were purchased from the Experimental Animal Center of Guangdong Province (Guangdong, China), and reared under specific pathogen-free conditions. Nude mice were intraperitoneally injected with LAD cells (1 × 106 cells/200 μL/mouse) transfected with sh-NC and oe-NC, with sh-HOXA10 and oe-NC, in addition to sh-HOXA10 and oe-HDAC1. The longest diameter (L) and shortest diameter (W) of the tumors in the mice were measured every 4 days post injection with the tumor growth plotted and a data curve constructed based on the formula V = L × W2 × 0.5. On day 25, the mice were euthanized and their tumors were excised. Six tumor samples from each group were weighed and averaged.

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