Glutathione S-transferase-fused DNMT1 was co-transfected into A549 cells with either sh-NC or sh-HDAC1, and 50 μL acetyltransferase assay buffer was incubated in 20 μM acetyl-CoA at 30 °C for 2 h. The reaction mixture was analyzed by SDS-PAGE and further examined through Western blot analysis.

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