The specimens of brain and cerebellum were fixed in 10% buffered formaldehyde. Then, the sample was dehydrated, embedded and sectioned. Sections (3.5 μm) were used for immunohistochemical staining, HE staining and Myelin staining later. In immunohistochemical staining, the primary antibodies included anti-NF-κB p65 antibody and anti-TNF-α antibody. Furthermore, we made the Myelin staining with Luxol Fast Blue/Cresyl Violet Stain Kit (G3245; Solarbio, Beijing, China) according to the manufacturer’s instruction. All the other chemical reagents used in this study were of analytical grade.

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