ChIP assays were performed using a ChIP kit (CST, USA) to investigate the intracellular combination between HIF1 and HREs of MAPKAPK5-AS1 promoter. Cells were crosslinked with formaldehyde and sonicated to an average length of 200–1000 bp. Then, Immunoprecipitation was conducted with an anti-HIF-1α antibody (Abcam) or IgG control. Precipitated DNA was amplified by qRT-PCR using primers listed in Additional File 1: Table S1.

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