Flow cytometry analysis of cell cycle and apoptosis

Cell cycle analysis was performed using cells that were treated with antibody to 5-bromo-2′-deoxyuridine-fluorescein isothiocyanate (FITC) (BD Biosciences, Franklin Lakes, NJ, USA) and DNA was stained with 7-AminoactinomycinD (7-ADD, Sigma-Aldrich Chemical Company, St Louis, MO, USA) [15].

Cell apoptosis analysis was performed in line with an Annexin V-FITC kit (Shanghai Beyotime Biotechnology Co., Ltd., Shanghai, China) which was used for staining, with both the DNA content and apoptosis analyzed using a FACScan flow cytometer (BD Biosciences).

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