The wild-type (wt) or mutant (mut) sequence of MAPKAPK5-AS1 or 3’UTR of PLAGL2 was cloned into pGL3 vector (Promega, Madison, WI, USA), separately. The wt or mut luciferase plasmids and miRNA were co-transfected into HEK293T cells. The MAPKAPK5-AS1 promoters containing different HREs are cloned into pGL3-basic vector, and the pGL3-MAPKAPK5-AS1vectors were co-transfected with si-control or si-HIF-1α under hypoxia. According to the manufacturer’s instructions, the luciferase activity was detected with a Dual-Luciferase Reporter Assay System (Promega, USA) 48 h after transfection.

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