The potential crude SlFNSI enzymes were incubated together with 160 μM α-oxoglutarate, 50 μM ferrous sulfate, and 200 μM eriodictyol in a final volume of 100 μl of PBS buffer (pH 7.0) for 1 h at 30 °C. The reaction was stopped by the addition of 400 μl of methanol. The mixture was then centrifuged at 20,000 g at 4 °C for 10 min after which the supernatant was collected for measurements.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.