The potential crude SlFNSI enzymes were incubated together with 160 μM α-oxoglutarate, 50 μM ferrous sulfate, and 200 μM eriodictyol in a final volume of 100 μl of PBS buffer (pH 7.0) for 1 h at 30 °C. The reaction was stopped by the addition of 400 μl of methanol. The mixture was then centrifuged at 20,000 g at 4 °C for 10 min after which the supernatant was collected for measurements.

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