SH-SY5Y cells were seeded on a 96-well plate at a density of 3 × 104 cells per well in 1:1 DMEM:F12 with phenol red, 4 mM glutamine, 200 U/ml penicillin, 200 U/ml streptomycin, MEM non-essential amino acids (100×; Gibco), and 10% FBS and incubated at 37 °C for 24 h, reaching up to 100% confluency, with 5% CO2. After 24 h, the medium was removed and replaced with 100 μl/well medium without phenol-red, containing 2% FBS and with 10 μM Aβ1–42 oligomers, and/or 1 μM CERTL, or alone to control wells and incubated for 24 h. Ten microliters of MTT (4 mg/ml) was added to each well and incubated at 37 °C for 3 h. MTT solution was decanted and the formazan was extracted with 100 μl of 4:1 DMSO:EtOH. Plates were read at 570 nm, with a reference filter at 690 nm.

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