All chemicals were obtained from Sigma-Aldrich unless stated otherwise. MCF10ADCIS.com cells (CVCL_5552, Asterand, Inc. Detroit, MI) [26, 27] were stably transfected with firefly luciferase and RFP [28] (herein named MCF10ADCIS), cultured in DMEM/F12 (1:1) medium supplemented with 5% horse serum (Thermo Fisher Scientific) and 4 mM glutamine, in a 37 °C humidified incubator with an atmosphere of 5% CO2. The cell line, which tested negative for mycoplasma by an RNA capture ELISA-based method, was genotyped upon arrival and used within 4 to 8 passages from the original stocks. An automated cell viability analyzer (Vi-Cell™, Beckman Coulter) was used to monitor cell number and viability. Where required, cell death was induced by incubating cells with 10 μM doxorubicin or 100 μM etoposide (Teva, Ltd.) for 24 h at 37 °C.

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