The MicroTom Metabolic Network (MMN), a high-temporal-resolution transcriptome and metabolome dataset that contains data from 20 different tissues and stages during the MicroTom growth cycle, was used to study the expression patterns of 2ODDs ( [25]. A heatmap of 2ODD expression was created and displayed using the R language program. Transcript abundance was calculated as fragments per kilobase of exon model per million mapped reads, and the resulting values were z-score transformed to normalize the gene expression levels.

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