Cells were added in a 96-well plate at a density of 1 × 104/ml. 20 μl MTT solution (Sigma, St. Louis, MO, USA) was added to each well after incubation for 24, 48, 72, and 96 h. Then, the cells continued to be cultured for 4 h in a humidified incubator. After the supernatants were removed, 200 μl DMSO was added to each well. Finally, the absorbance was detected with a microplate reader (Bio-Rad, Hercules, CA, USA).

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